Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation |
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Authors: | Les A. Edye Michael R. Johns Ken N. Ewings |
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Affiliation: | (1) Department of Microbiology, University of Queensland, 4067 St. Lucia, Australia;(2) Department of Chemical Engineering, University of Queensland, 4067 St. Lucia, Australia;(3) Queensland Science and Technology Ltd., Milton, Brisbane, Australia |
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Abstract: | Summary Fed-batch cultures of Zymomonas mobilis (UQM 2864), a mutant unable to metabolise fructose, grown on diluted sugar cane syrup (200 g/l sucrose) achieved yields of 90.5 g/l fructose and 48.3 g/l ethanol with minimal sorbitol formation and complete utilization of the substrate. The effect of inoculum size on sorbitol formation in the batch stage of fed-batch fermentation are reported. Fermentation of sucrose (350 g/l) supplemented with nutrients yielded 142 g/l fructose and 76.5 g/l ethanol. Some fructose product loss at high fructose concentrations was observed. The fed-batch fermentation process offers a method for obtaining high concentrations of fructose and ethanol from sucrose materials. |
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