Production of monoclonal antibodies for the detection of potato virus Y |
| |
| Authors: | D G ROSE A L HUBBARD† |
| |
| Institution: | *Department of Agriculture &Fisheries for Scotland, Agricultural Scientific Services, East Craigs, Edinburgh, EH12 8NJ;?Clinical and Population Cytogenetics Unit, Medical Research Council, Western General Hospital, Edinburgh, EH4 2XU |
| |
| Abstract: | Monoclonal antibodies (McAb) were obtained to potato virus Y (PVY) after immunisation of BALB/c mice with purified PVY, tobacco necrotic strain (PVYn). Spleen cells from a mouse showing a high serum titre were used for fusion with X63NS1 myeloma cells. Hybridomas were selected in medium containing HAT. Culture supernatants were screened for antibody production against PVY, ordinary strain (PVY0) and PVYn using indirect ELISA. Clones of interest were further cross-reacted with 12 isolates each of PVY0 and PVYn and two isolates of potato virus A (PVA) and healthy sap. For further trials, two clones which reacted specifically with PVYn isolates and one which detected all PVY isolates except two of potato virus C (PVC) were selected. |
| |
| Keywords: | |
|
|
