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Vasoactive intestinal polypeptide binds with high affinity to non-small cell lung cancer cells and elevates cyclic AMP levels |
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| Authors: | M Lee RT Jensen SC Huang G Bepler L Korman TW Moody |
| Institution: | * Department of Biochemistry and Molecular Biology The George Washington University School of Medicine and Health Sciences, Washington, DC 20037, USA ? Digestive Diseases Branch, National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Bethesda, MD 20892, USA ? Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA § Gastroenterology Section, Veterans Administration Medical Center, Washington, DC 20422, USA |
| Abstract: | Vasoactive intestinal polypeptide (VIP) receptors were characterized on non-small cell lung cancer (NSCLC) cells. 125I-VIP bound specifically to membranes derived from 6 NSCLC cell lines. Specific 125I-VIP was time dependent and a linear function of EPLC-65H membrane concentration. 125I-VIP bound with high (Kd=0.2 nM) and moderate (Kd=39 nM) affinity to two classes of sites. Pharmacology studies indicated that the order of peptide potency was VIP > rGHRH > PHI = helodermin > secretin > glucagon. Also VIP elevated the cAMP levels 10-fold using cell line ADLC-5M2. These data indicate that functional VIP receptors are present on NSCLC cells. |
| Keywords: | VIP VIP receptors Non-small cell lung cancer Cyclic AMP |
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