Identification of 36 kDa phosphoprotein in fibrous sheath of hamster spermatozoa |
| |
| Authors: | Fujinoki Masakatsu Kawamura Takeshi Toda Toshifusa Ohtake Hideki Ishimoda-Takagi Tadashi Shimizu Nobuyoshi Yamaoka Sadao Okuno Makoto |
| |
| Institution: | a Department of Physiology, Dokkyo University School of Medicine, 880 kitakobayasi, Mibu, Tochigi 321-0293, Japan;b Department of Molecular Biology, Keio University School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan;c TMIG Proteomics Collaboration Center, Tokyo Metropolitan Institute of Gerontology, Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan;d Department of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan;e Department of Life Sciences, Graduate School of Arts and Science, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-0041, Japan |
| |
| Abstract: | In our previous studies (Fujinoki et al., 2001, 2003), we reported that two types of 36 kDa proteins, designated 36K-A protein and 36K-B protein, obtained from hamster sperm flagella, are associated with motility activation and phosphorylated in a cAMP-dependent manner at serine residues. In the present experiments, we focused on the hamster (Mesocricetus auratus) 36K-A protein, which was analyzed by peptide mass finger printing and amino acid sequencing. The results suggest that 36K-A protein is a pyruvate dehydrogenase E1 component β subunit lacking the N-terminal 30 amino acids. Moreover, our results suggest that 36 K-A protein is localized in the fibrous sheath of the principal piece of hamster spermatazoa. |
| |
| Keywords: | Author Keywords: Fibrous sheath Flagellum Hamster LC-MS/MS Peptide mass finger printing Phosphorylation Pyruvate dehydrogenase Spermatozoa |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
