白色念珠菌体外诱导氟康唑耐药性的实验研究（简报）

随着广谱抗生素的普遍应用以及免疫缺陷人群的增加，机会性致病菌念珠菌感染日益增多。深部念珠菌感染已经成为重症患者死亡的重要原因，白色念珠菌(C．albicans)是其中的主要致病菌。低毒广谱的唑类抗真菌药氟康唑是既能治疗严重真菌感染又不会产生明显副作用的少数抗真菌药之一，它的广泛使用取得良好治疗效果，但也导致菌株耐药率增加而使临床治疗失败。近10年来，在治疗

Study on experimental induction of fluconazole resistance in Candida albicans]

To investigate the phenotype and genotype variation between the Fluconazole resistant C. albicans isolates and the corresponding susceptible ones, our research established a resistance-induction mode in vitro. Comparisons were done on drug resistance maintainability, metabolic profile and the doubling time in the logarithmic growth phase. Genotypes were determined by ERIC-PCR. The Fluconazole resistant isolates appeared in strain 435, A06, B07 and C01 from total 22 clinical Fluconazole susceptible isolates after being incubated for 45-80 days in YEPD broth with increasing Fluconazole concentration. The parent isolates had a same metabolic profile and a similar growth doubling time to their filial generation. The same ERIC-PCR profiles were also found between the susceptible parents and their resistant filial isolates. The resistant isolates maintained drug resistance for 24 days after growing on drug-free medium. It was supposed that candida albicans had a latent capacity to evolve resistance to azoles under a certain antifungal drug selective pressure, and the acquired resistance could maintain in drug-free media for a certain period. The resistant isolate with no adaptive cost may be prone to vogue among people. ERIC-PCR could be used in epidemiological study as a stable marker.