非损伤性取样在朱?种群遗传研究中的应用

采用非损伤性取样方法提取DNA，利用20条10bp的随机引物对陕西朱?保护观察站的16只朱?个体间遗传距离进行分析研究。获得的DNA可进行RAPD研究分析，18条引物扩增结果稳定，遗传距离指数介于30.52%～10.18%之间；揭示16只朱?间亲缘关系较近。尝试在朱?种群遗传学研究中应用非损伤性方法抽提基因组DNA，以方便对朱?等小种群珍稀濒危生物进行相关研究。Abstract：Genetic distance of sixteen crested ibises (Nipponia nippon) was analyzed at the DNA level by polymerase chain reaction (PCR) using random primers after DNA was sampled with non-invasive sampling. The amount of DNA was enough to apply t o genetic analysis by RAPD. Eighteen primers had stable effect. The genetic dist ances are between 30.52% and 10.18%，which showed that the relationship between 16 crested ibises was similar. This study tried to apply a non-invasive method to obtain DNA, which is helpful to research on the genetic background of creste d ibises and other tiny population based on DNA level.

Application of theNon-invasive Sampling to Crested Ibises

Genetic distance of sixteen crested ibises ( Nipponia nippon ) was analyzed at the DNA level by polymerase chain reaction (PCR) using random primers after DNA was sampled with non-invasive sampling. The amount of DNA was enough to apply to genetic analysis by RAPD. Eighteen primers had stable effect. The genetic distances are between 30 52% and 10.18%,which showed that the relationship between 16 crested ibises was similar. This study tried to apply a non-invasive method to obtain DNA, which is helpful to research on the genetic background of crested ibises and other tiny population based on DNA level.