Proteolytic activity in electropherograms (SDS-PAGE) of bovine erythrocyte ghosts |
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| Authors: | M Gaczynska |
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| Affiliation: | Laboratory of Biophysics of Development and Aging, University of Lód?, Poland. |
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| Abstract: | 1. Activity of proteases, strongly related with erythrocyte membrane, was analysed employing a new methodological approach. 2. Intact bovine ghosts, ghosts depleted in peripheric proteins or purified Triton X-100 and ghost extracts were electrophoresed and the proteolytic activity in the gel fragments (SDS-PAGE) was assayed. 3. At least two proteases that were inhibited by EDTA and PMSF were found. |
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