Hydrolysis of linear DNA duplex catalyzed by Co(III) complex of cyclen attached to polystyrene. |
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Authors: | C S Jeung J B Song Y H Kim J Suh |
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Affiliation: | Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, Republic of Korea. |
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Abstract: | To design artificial restriction enzymes, synthetic catalytic centers that effectively hydrolyze linear double-stranded polydeoxyribonucleotides are needed. The Co(III) complex of cyclen (CoCyc) attached to polystyrene derivatives hydrolyzes linearized pUC18 DNA with half-lives as short as 30 min at 25 degrees C. The catalytic activity of CoCyc is enhanced by >150 times on attachment to the resin. |
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