DNA mediated transformation of Aspergillus ficuum |
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Authors: | Edward J Mullaney Peter J Punt Cees A M J J Van den Hondel |
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Institution: | (1) Southern Regional Research Center, ARS, USDA, P. O. Box 19687, 70179 New Orleans, LA, USA;(2) Medical Biological Laboratory, TNO, P. O. Box 45, NL 2280 AA Rijswijk, The Netherlands |
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Abstract: | Summary Two DNA-mediated transformation systems were successfully adapted to Aspergillus ficuum. Both the Escherichia coli hygromycin B resistance gene and the A. nidulans amdS gene transformation systems produced stable A. ficuum NRRL 3135 transformants. Cotransformation with the E. coli lacZ gene was also achieved with the hygromycin B system. In cotransformation a second unselected gene, in this case the lacZ gene which codes for -galactosidase, was also integrated and expressed in hygromycin B transformants. Since both of these transformation systems utilized dominant selection markers, they are potentially useful in other genetically uncharacterized filamentous fungi. |
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