Surface expression of <Emphasis Type="Italic">Helicobacter pylori</Emphasis> urease subunit B gene E fragment on <Emphasis Type="Italic">Lactococcus lactis</Emphasis> by means of the cell wall anchor of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> protein A |
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Authors: | Dafeng Song Qing Gu |
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Institution: | (1) Department of Biotechnology, Zhejiang Gongshang University, 310035 Hangzhou, China;(2) Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, 310012 Hangzhou, China |
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Abstract: | Fragment E of ureB (ureBE) was cloned from a clinical isolate of Helicobacter pylori. A prokaryotic expression vector, pAMJ399, with the ureB fragment E and the Staphylococcus aureus protein A anchor fragment (spaX), was constructed. The fusion protein was expressed under the control of the P170 promoter in Lactococcus lactis. Western blot assay of lactococcal cell wall extracts with a polyclonal chicken antiserum confirmed the immunity of the expressed
recombinant protein which was located on the cell surface. These results provide the first report of a surface display system
in lactic acid bacteria for the delivery of oral vaccines against Helicobacter pylori. |
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Keywords: | Helicobacter pylori Lactococcus lactis Staphylococcus aureus protein A anchor fragment Urease subunit B Vaccine |
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