Isolation and characterization of four somatic embryogenesis receptor-like kinase (<Emphasis Type="Italic">RhSERK</Emphasis>) genes from miniature potted rose (<Emphasis Type="Italic">Rosa hybrida</Emphasis> cv. Linda) |
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Authors: | Hedayat Zakizadeh Bjarne M Stummann Henrik Lütken Renate Müller |
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Institution: | (1) Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Hojbakkegaard Alle 21, 2630 Taastrup, Denmark;(2) Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, P. O. Box 41635-1314, Rasht, Iran |
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Abstract: | The isolation and expression analysis of four partial gene sequences from rose (Rosa hybrida cv. Linda) belonging to the receptor-like kinase gene superfamily are reported. These genes have been designated RhSERK1 to RhSERK4 (Accession No. EF631967 to EF631970) as they exhibit high sequence identities with genes from the somatic embryogenesis receptor-like
kinase (SERK) family in other plant species. The RhSERK genes are differentially expressed in non-embryogenic callus, embryogenic callus, mature somatic embryos and a range of tissues
from intact plants, indicating a broad role in plant growth and development. However, the expressions of RhSERK3 and RhSERK4 were approximately fivefold higher in embryogenic callus than in non-embryogenic callus, and they are even higher when compared
to tissues from intact plants. In addition, RhSERK4 expression was approximately eightfold higher in somatic embryos than in embryogenic callus. These results suggest that the
expression pattern of RhSERK3 and RhSERK4 may be used as a marker of somatic embryogenesis. |
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