Infection byBeauveria bassiana ofLeptinotarsa decemlineata larvae as a consequence of fecal contamination of the integument followingper os inoculation |
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| Authors: | Leslie L Allee Mark S Goettel Alla Gol'berg H S Whitney D W Roberts |
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| Institution: | (1) Insect Pathology Resource Center, Boyce Thompson Institute, Cornell University, Tower Road, 14853 Ithaca, NY, USA;(2) Agricultural Research Organization, Gilat Experiment Station, Mobile Post Negev 2, 85-280, Israel;(3) Pacific Forestry Centre, Forestry Canada, 506 West Burnside Road, V8Z 1M5 Victoria, British Columbia, Canada;(4) Present address: Crop Sciences Section, Research Station, Research Branch, Agriculture Canada, T1J 4B1 Lethbridge, Alberta, Canada;(5) Boyce Thompson Institute, Tower Rd., 14853 Ithaca, NY, USA |
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| Abstract: | A study ofper os inoculated larvae was undertaken to determine if the entomopathogenic fungus,Beauveria bassiana, is able to infect the Colorado potato beetle,Leptinotarsa decemlineata, via the alimentary tract. Surface orper os inoculated larvae which were immediately surface sterilized post inoculation did not succumb to infection, whereas those larvae not sterilized became infected. Histological studies of fed or starved, agnotobiotic (with microbial flora) and axenic larvae revealed that conidia can germinate in the gut regardless of the presence of gut microflora. However, infection via the alimentary tract was never observed in fed larvae and only noted in a single starved individual. It is concluded that infections ofper os inoclated larvae occurred after surface contamination of the integument by viable conidia contained in the frass. The rate of food passage through the gut is probably important in preventingper os infections. |
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| Keywords: | Beauveria bassiana entomopathogenic fungus gut infection histopathology Leptinotarsa decemlineata pathogenesis |
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