| 黑木耳亲本、单核、杂交菌株鉴定的研究 |
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| 引用本文： | 韩增华,张丕奇,戴肖东,孔祥辉,马庆芳,张介驰. 黑木耳亲本、单核、杂交菌株鉴定的研究[J]. 菌物研究, 2009, 7(2): 99-103 |
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| 作者姓名： | 韩增华 张丕奇 戴肖东 孔祥辉 马庆芳 张介驰 |
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| 作者单位： | 黑龙江省科学院微生物研究所,哈尔滨,150010;黑龙江省科学院微生物研究所,哈尔滨,150010;黑龙江省科学院微生物研究所,哈尔滨,150010;黑龙江省科学院微生物研究所,哈尔滨,150010;黑龙江省科学院微生物研究所,哈尔滨,150010;黑龙江省科学院微生物研究所,哈尔滨,150010 |
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| 基金项目： | 黑龙江省科学院创新基金项目 |
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| 摘 要： | 应用荧光核染色技术、酯酶同工酶鉴定技术,对黑木耳亲本双核菌株、原生质体单核菌株和单-单杂交菌株进行筛选鉴定。结果表明:荧光核染色技术可快速有效地鉴定获得菌株的核相,酯酶同工酶技术可准确地区分单核菌株及杂交新菌株的不同核型,研究得到1个酶带差异显著的新菌株。
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| 关 键 词： | 黑木耳 原生质体单核 杂交菌株 荧光核染色 酯酶同工酶 菌株鉴定 |
Study on Identification of Parent Monocaryon and Hybrid Stains in Auricularia auricula |
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| HAN Zeng-hua,ZHANG Pi-qi,DAI Xiao-dong,KONG Xiang-hui,MA Qing-fang,ZHANG Jie-chi. Study on Identification of Parent Monocaryon and Hybrid Stains in Auricularia auricula[J]. Journal of Fungal Research, 2009, 7(2): 99-103 |
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| Authors: | HAN Zeng-hua ZHANG Pi-qi DAI Xiao-dong KONG Xiang-hui MA Qing-fang ZHANG Jie-chi |
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| Affiliation: | ( Institute of Microbiology, Heilongfiang Academy of Sciences, Harbin 150010, China ) |
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| Abstract: | The parent, monocaryon and hybrid stains of Auricularia auricula were identyfied using the techneque of esterase isozyme and nuclear fluorescence staining. The result showed that nuclear fluorescence staining technology was an effective way to identify nuclear number of the strains and esterase isozyme analysis can quickly and accurately classify hybrid strain with a marketed difference was obtained the parent, monocaryon and hybrid strains. A new through the study. |
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| Keywords: | Auricularia auricula protoplast monocaryons hybrid strain nuclear fluorescence staining esterase isozyme zymogram strain identification |
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