Screening and characterization of endophytic fungi of Panax ginseng Meyer for biocontrol activity against ginseng pathogens |
| |
| Institution: | 1. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;2. Department of Orthopedic Surgery, School of Medicine, Ajou University, Suwon 442-749, Republic of Korea;3. Punggi Ginseng Experiment Station, Gyeongbuk Provincial Agricultural Research & Extension Services, Yeongju, Gyeongbuk 750-870, Republic of Korea;4. Division of Forest Pests & Diseases, Korea Forest Research Institute, Seoul 130-712, Republic of Korea;5. College of Pharmacy, Chungang University, Seoul 156-756, Republic of Korea;6. Department of Horticulture, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;1. School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada;2. Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada;1. College of Life Science, Jilin Agricultural University, Changchun 130118, PR China;2. College of Plant Protection, Jilin Agricultural University, Changchun 130118, PR China;1. Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea;2. Natural and Medical Sciences Research Center, University of Nizwa, Nizwa 616, Oman;3. Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea;4. Crop Biotechnology Institute, Green Bio Science & Technology, Seoul National University, Pyeongchang, Kangwon 25354, Republic of Korea;5. Natural Constituents Research Center, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea;1. Agricultural Biotechnology & Phytopathology Laboratory, Department of Botany, University of Karachi, Karachi, 75270, Pakistan;2. Postharvest & Food Biochemistry Laboratory, Department of Food Science & Technology, University of Karachi, Karachi, 75270, Pakistan;3. Biotechnology & Drug Development Laboratory, Department of Biochemistry, University of Karachi, Karachi, 75270, Pakistan;4. H.E.J. Research Institute of Chemistry & International Center for Chemical & Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan;1. Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea;2. Division of Agricultural Microbiology, National Academy of Agricultural Sciences, Rural Development Administration, Wanju, Korea;3. Department of Biology, Chungbuk National University, Cheongju, Chungbuk, Korea |
| |
| Abstract: | Forty endophytic fungi isolated from ginseng plants were screened to identify metabolites that had antifungal activity against ginseng microbial pathogens. The metabolites from the fungi were extracted from the liquid culture filtrates using ethyl acetate and then evaluated in vitro for antimicrobial activity against ginseng pathogens (Alternaria panax, Botrytis cinerea, Colletotrichum panacicola, Cylindrocarpon destructans, Rhizoctonia solani, and Phytophthora cactorum). Six of the fungi (Colletotrichum pisi, Fusarium oxysporum, Fusarium solani, Phoma terrestris, unknown 1 and 2) showed effective antimicrobial activity against all or some of the ginseng pathogens, with the extract of P. terrestris showing the strongest antimicrobial activity. The extract also showed inhibitory activity against spore germination of the pathogens. Gas chromatography–mass spectrometry (GC–MS) analysis of P. terrestris extract revealed that forty-one compounds were present in metabolites containing mainly N-amino-3-hydroxy-6-methoxyphthalimide (32% of the total metabolites) and 5H-dibenz B, F] azepine (7%). Treatment with P. terrestris extract also caused morphological changes and reduced expression of the genes involved in mycelial growth and virulence. Treatment also induced defense-related genes in detached Arabidopsis leaves that were inoculated with the pathogens. These results indicate the antimicrobial potential for use of metabolites extracted from the ginseng endophytic fungi as alternatives to chemicals for biocontrol. |
| |
| Keywords: | Endophytic fungi Antimicrobial metabolites Gas chromatography–mass spectrometry (GC–MS) Scanning electron microscopy (SEM) |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
