Microbial acetyl-CoA metabolism and metabolic engineering |
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| Institution: | 1. Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main St, Houston, TX 77005, USA;2. Department of Bioengineering, Rice University, 6100 Main St, Houston, TX 77005, USA;3. Department of Chemistry, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;4. Biochemistry & Molecular Medicine, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;5. Genome Center, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;1. Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea;2. Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon 34113, Republic of Korea;3. Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, Jinju 52828, Republic of Korea;1. Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS-362, Houston, TX 77005, USA;2. Department of Bioengineering, Rice University, 6100 Main Street, MS-142, Houston, TX 77005, USA |
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| Abstract: | Recent concerns over the sustainability of petrochemical-based processes for production of desired chemicals have fueled research into alternative modes of production. Metabolic engineering of microbial cell factories such as Saccharomyces cerevisiae and Escherichia coli offers a sustainable and flexible alternative for the production of various molecules. Acetyl-CoA is a key molecule in microbial central carbon metabolism and is involved in a variety of cellular processes. In addition, it functions as a precursor for many molecules of biotechnological relevance. Therefore, much interest exists in engineering the metabolism around the acetyl-CoA pools in cells in order to increase product titers. Here we provide an overview of the acetyl-CoA metabolism in eukaryotic and prokaryotic microbes (with a focus on S. cerevisiae and E. coli), with an emphasis on reactions involved in the production and consumption of acetyl-CoA. In addition, we review various strategies that have been used to increase acetyl-CoA production in these microbes. |
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| Keywords: | Yeast Bacteria Acetyl-CoA Central carbon metabolism Industrial biotechnology |
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