Complete genome sequence,metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic,fast growing,xylose-utilizing bacterium |
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| Affiliation: | 1. Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China;2. Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Fudan University, Shanghai, China;3. Department of Interventional Radiology, Zhongshan Hospital, Fudan University, China;4. Department of Radiology, Zhongshan Hospital, Fudan University, China;5. Institute of Biomedical Sciences, Fudan University, Shanghai, China;6. State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China;7. Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, HKSAR, China;1. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, United States;2. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, United States;3. Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee – Knoxville, Knoxville, TN 37996, United States;1. University of Salford, Salford, UK;2. Liverpool Vascular & Endovascular Service, Liverpool, UK;3. Royal Liverpool & Broadgreen University Hospital, Liverpool, UK;1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea;2. BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea;3. BioInformatics Research Center, KAIST, Daejeon 305-701, Republic of Korea;4. The Novo Nordisk Foundation Center for Biosustainability, Technical University Denmark, Hørsholm, Denmark |
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| Abstract: | We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h−1 on glucose and 1.52 h−1 on xylose (doubling time less than 30 min). The corresponding specific glucose and xylose utilization rates are 5.55 g/g/h and 5.24 g/g/h, respectively. As such, Geobacillus LC300 grows 3-times faster than E. coli on glucose and xylose, and has a specific xylose utilization rate that is 3-times higher than the best metabolically engineered organism to date. To gain more insight into the metabolism of Geobacillus LC300 its genome was sequenced using PacBio׳s RS II single-molecule real-time (SMRT) sequencing platform and annotated using the RAST server. Based on the genome annotation and the measured biomass composition a core metabolic network model was constructed. To further demonstrate the biotechnological potential of this organism, Geobacillus LC300 was grown to high cell-densities in a fed-batch culture, where cells maintained a high xylose utilization rate under low dissolved oxygen concentrations. All of these characteristics make Geobacillus LC300 an attractive host for future metabolic engineering and biotechnology applications. |
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| Keywords: | Thermophile Genome analysis Metabolic network model Xylose metabolism High-cell-density fermentation |
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