RBM5 and p53 expression after rat spinal cord injury: Implications for neuronal apoptosis |
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| Institution: | 1. Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, Jiangsu, People''s Republic of China;2. Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, Nantong University, 226001 Nantong, Jiangsu, People''s Republic of China;1. University of Alabama Birmingham, Department of Psychiatry & Behavioral Neurobiology, United States;2. University of Alabama Birmingham, Department of Neurobiology, Evelyn F. McKnight Brain Institute, Civitan International Research Center, United States;1. Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China;2. Breast Surgery Department, Chinese Academy of Medical Sciences & Peking Union Medical College, Peking Union Medical College and Hospital, China;1. INRA, UMR1198 Biologie du Développement et Reproduction, Département de Physiologie Animale et Systèmes d’Elevage, F-78350 Jouy-en-Josas, France;2. Department of Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran;3. Unité de Biochimie Hormonale et Nutritionnelle, Centre Hospitalier Universitaire de Grenoble, Département de Biologie – Toxicologie – pharmacologie, 38043 Grenoble cedex 9, France;4. Institute of Medical Sciences, Division of Applied Medicine, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK;1. Department of Molecular Medicine, College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China;2. Research Center of Cancer Prevention & Treatment, Translational Medicine Research Center of Liver Cancer, Hunan Provincial Tumor Hospital (Affiliated Tumor Hospital of Xiangya Medical School of Central South University), Changsha 410013, China;1. Department of Hematology, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Université Sorbonne Paris Cité, 125 rue de Stalingrad, 93000, Bobigny, France;2. Department of Pathology, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Université Sorbonne Paris Cité, 125 rue de Stalingrad, 93000, Bobigny, France;3. Hematology Laboratory, Biology and Pathology Center, Lille University Hospital, Lille, France;4. Hematology Laboratory, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Université Sorbonne Paris Cité, 125 rue de Stalingrad, 93000, Bobigny, France;5. Hématologie Seniors, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Université Sorbonne Paris Cité, 1 avenue Claude Vellefaux, 75010, Paris, France |
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| Abstract: | RBM5 (RNA-binding motif protein 5), a nuclear RNA binding protein, is known to trigger apoptosis and induce cell cycle arrest by regulating the activity of the tumor suppressor protein p53. However, its expression and function in spinal cord injury (SCI) are still unknown. To investigate whether RBM5 is involved in central nervous system injury and repair, we performed an acute SCI model in adult rats in this study. Our results showed RBM5 was unregulated significantly after SCI, which was accompanied with an increase in the levels of apoptotic proteins such as p53, Bax, and active caspase-3. Immunofluorescent labeling also showed that traumatic SCI induced RBM5 location changes and co-localization with active caspase-3 in neurons. To further probe the role of RBM5, a neuronal cell line PC12 was employed to establish an apoptotic model. Knockdown of RBM5 apparently decreased the level of p53 as well as active caspase-3, demonstrating its pro-apoptotic role in neurons by regulating expressions of p53 and caspase-3. Taken together, our findings indicate that RBM5 promotes neuronal apoptosis through modulating p53 signaling pathway following SCI. |
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| Keywords: | RBM5 Spinal cord injury Neuron Apoptosis p53 |
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