Regulation of Lactobacillus plantarum contamination on the carbohydrate and energy related metabolisms of Saccharomyces cerevisiae during bioethanol fermentation |
| |
Affiliation: | 1. The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800, Kongens Lyngby, Denmark;2. School of Chemical Engineering, University of Campinas, P.O. Box 6066, 13081-970, Campinas, São Paulo, Brazil;3. Brazilian Bioethanol Science and Technology Laboratory/Brazilian Center of Research in Energy and Materials (CTBE/CNPEM), P. O. Box 6192, 13083-970, Campinas, São Paulo, Brazil;4. Department of Engineering and Computer Science, Andrews University, Berrien Springs, MI, USA;5. Interdisciplinary Center of Energy Planning (NIPE), University of Campinas, P. O. Box 6166, 13083-896, Campinas, São Paulo, Brazil;1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People’s Republic of China;2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People’s Republic of China;3. Research Centre of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, People’s Republic of China;1. Dipartimento di Scienze Agrarie, degli Alimenti e dell''Ambiente, Università di Foggia, via Napoli 25, 71122, Foggia, Italy;2. Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, Unità Operativa di Supporto di Lecce, Lecce, Italy;3. ENOLAB – Laboratori de Microbiologia Enològica, ERI/ISIC BioTecMed, Universitat de València, Spain;4. Promis Biotech srl, via Napoli 25, 71122, Foggia, Italy |
| |
Abstract: | During the industrial bioethanol fermentation, Saccharomyces cerevisiae cells are often stressed by bacterial contaminants, especially lactic acid bacteria. Generally, lactic acid bacteria contamination can inhibit S. cerevisiae cell growth through secreting lactic acid and competing with yeast cells for micronutrients and living space. However, whether are there still any other influences of lactic acid bacteria on yeast or not? In this study, Lactobacillus plantarum ATCC 8014 was co-cultivated with S. cerevisiae S288c to mimic the L. plantarum contamination in industrial bioethanol fermentation. The contaminative L. plantarum-associated expression changes of genes involved in carbohydrate and energy related metabolisms in S. cerevisiae cells were determined by quantitative real-time polymerase chain reaction to evaluate the influence of L. plantarum on carbon source utilization and energy related metabolism in yeast cells during bioethanol fermentation. Contaminative L. plantarum influenced the expression of most of genes which are responsible for encoding key enzymes involved in glucose related metabolisms in S. cerevisiae. Specific for, contaminated L. plantarum inhibited EMP pathway but promoted TCA cycle, glyoxylate cycle, HMP, glycerol synthesis pathway, and redox pathway in S. cerevisiae cells. In the presence of L. plantarum, the carbon flux in S. cerevisiae cells was redistributed from fermentation to respiratory and more reducing power was produced to deal with the excess NADH. Moreover, L. plantarum contamination might confer higher ethanol tolerance to yeast cells through promoting accumulation of glycerol. These results also highlighted our knowledge about relationship between contaminative lactic acid bacteria and S. cerevisiae during bioethanol fermentation. |
| |
Keywords: | Bioethanol Bacterial contamination Metabolic regulation alcohol dehydrogenase aldehyde dehydrogenase citrate synthase nonmitochondrial citrate synthase EMP" },{" #name" :" keyword" ," $" :{" id" :" kw0085" }," $$" :[{" #name" :" text" ," _" :" Embden-Meyerhof-Pamas glycerol 2-dehydrogenase glycerol-3-phosphate dehydrogenase glycerol-1-phosphatase HMP" },{" #name" :" keyword" ," $" :{" id" :" kw0125" }," $$" :[{" #name" :" text" ," _" :" hexose monophosphate pathway hexokinase 2 isocitrate lyase isocitrate dehydrogenase oxoglutarate dehydrogenase LAB" },{" #name" :" keyword" ," $" :{" id" :" kw0175" }," $$" :[{" #name" :" text" ," _" :" lactic acid bacteria malate synthase NADH dehydrogenase NADH dehydrogenase NADH dehydrogenase phosphofructokinase-1 pyruvate kinase qRT-PCR" },{" #name" :" keyword" ," $" :{" id" :" kw0245" }," $$" :[{" #name" :" text" ," _" :" quantitative real-time PCR QS" },{" #name" :" keyword" ," $" :{" id" :" kw0255" }," $$" :[{" #name" :" text" ," _" :" quorum sensing TCA" },{" #name" :" keyword" ," $" :{" id" :" kw0265" }," $$" :[{" #name" :" text" ," _" :" tricarboxylic acid cycle trifunctional aldehyde reductase 6-Phosphogluconolactonase |
本文献已被 ScienceDirect 等数据库收录! |
|