Myrothecium verrucaria strain X-16, a novel parasitic fungus to Meloidogyne hapla |
| |
| Institution: | 1. Agricultural College, Shanxi Agricultural University, Taigu 030801, China;2. AgriLife Research and Extension Center, Texas A&M University System, Beaumont 77713, USA;1. Sustainable Agricultural Systems Laboratory, 10300 Baltimore Ave, Bldg 001, Rm 123, Beltsville, MD 20705, United States;2. USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD, 20705, United States;1. Centre for Rural Development & Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India;2. Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India;1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China;2. Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, Nanjing University of Posts and Telecommunications, Nanjing 210023, China;3. Department of Biology, McMaster University, 1280 Main St. West, Hamilton, ON L8S 4K1, Canada;1. College of Life Science, Agriculture and Forestry, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, China;2. Department of Plant Pathology, The University of Agriculture Peshawar, 25000, Pakistan |
| |
| Abstract: | Options for control of northern root-knot nematode (NRKN, Meloidogyne hapla) on vegetables are very limited currently. In this study, we characterized the parasitism of Myrothecium verrucaria strain X-16, a new nematophagous fungus, on NRKN at the stages of eggs, J2, and adult females and evaluated its biocontrol efficacy in the greenhouse. Strain X-16 produced conidia that geminated and invaded in 80 h after in contact with eggs, causing the shrinkage and depression of egg shell and blastocolysis of the embryo. The strain also attacked 2nd-stage juveniles by producing developing surface networks of hyphae on the nematode body wall. Strain X-16 attacked adult females by producing dense networks of hyphae on the nematode body wall in 120 h. Strain X-16 had lethal effects (22–71% mortality) against NRKN J2 at the concentration as low as 3.1 × 107 conidia/ml and with the incubation treatment time as short as 24 h. The lethal effects linearly increased with the increase of conidial concentration, with the estimated LC50 values as low as 1.0 × 108 conidia/ml. Soil treatments with strain X-16 at 1%, 2% or 4% (wt/wt) induced significant reductions in J2 nematode counts in 100 g of dry soil, Pf/Pi ratios and root-know index in cucumber in the greenhouse evaluations. These studies are the first to demonstrate that M. verrucaria is able to parasitize NRKN and strain X-16 can be a potential biocontrol agent for management of NRKN. |
| |
| Keywords: | Parasitism Pathogenicity |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
