Gata4, Tbx5 and Baf60c induce differentiation of adipose tissue-derived mesenchymal stem cells into beating cardiomyocytes |
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| Institution: | 1. Advanced Heart Failure and Transplantation Center, UMC Ljubljana, Slovenia;2. Department of Hematology, UMC Ljubljana, Slovenia;3. National Blood Transfusion Institute, Ljubljana, Slovenia;4. Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA;1. Department of Cardiology, Medical University of Vienna, Austria;2. Department of Biomedical Laboratory and Imaging Science, Faculty of Medicine, University of Debrecen, Debrecen, Hungary;3. Exercise Biochemistry Laboratory, The University of Memphis, Department of Health and Sport Sciences, Memphis, TN, USA;4. Department of Cardiology, University of Medicine and Pharmacy Tirgu Mures, Romania;5. Internal Medicine, Mayo Graduate School of Medicine, Mayo Clinic, Rochester, MN, USA;6. rd |
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| Abstract: | The adipose tissue-derived mesenchymal stem cells (ADMSCs) are extensively utilized in tissue engineering, regenerative medicine and cell therapy. ADMSCs can differentiate into cardiomyocytes, and it has been shown that over-expression of a cocktail of factors can induce ectopic heart formation and program cardiogenesis in ESCs. However, which genes are responsible for differentiation of ADMSCs into beating cardiomyocyte-like cells remains unknown. In this study we have shown that the combination of Gata4, Tbx5 and Baf60c is sufficient for inducing ADMSCs to form cardiomyocytes. It also appears that, while Gata4 and Baf60c are key inducers of myocardial differentiation, Tbx5 is essential for the ability of cardiac cells to contract. These findings provide additional experimental references for myocardial tissue engineering in the emerging field of cell-based therapy of heart diseases. |
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| Keywords: | cTnT Cardiomyocytes differentiation Adipose derived mesenchymal stem cells |
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