Review of methods to probe single cell metabolism and bioenergetics |
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Affiliation: | 2. Department of Veterinary Biochemistry, Ranchi Veterinary College, Birsa Agricultural University, Ranchi, India;3. Department of Veterinary Physiology, COVAS, KVASU, Wayanad, India;4. Department of Veterinary Anatomy and Histology, COVAS, KVASU, Wayanad, India;5. Center for Medical Biotechnology, M.D. University, Rohtak, India;6. Department of Veterinary Physiology and Biochemistry, COVAS, SVP University of Agriculture and Technology, Meerut, India |
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Abstract: | Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed. |
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Keywords: | Single cell analysis Metabolism Bioenergetics Microfluidics Microscopy |
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