| 酶定向进化的研究进展 |
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| 引用本文： | 罗巅辉,方柏山.酶定向进化的研究进展[J].生物加工过程,2006,4(1):9-15. |
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| 作者姓名： | 罗巅辉 方柏山 |
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| 作者单位： | 华侨大学,工业生物技术研究所,泉州,362021 |
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| 基金项目： | 国家自然科学基金资助项目(20276026,20446004)，福建省自然科学基金资助项目(Z0516009)，福建省科技计划重点课题基金资助项目(2003I020) |
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| 摘 要： | 定向进化在改造酶的性质方面已得到广泛应用,各种建立突变库的方法不断涌现。对新近发展的几种突变技术(如寡核苷酸设计型装配重组技术ADO、非序列同源蛋白重组SHIPREC等)进行了简要地介绍与分类。与突变技术相对应的筛选方法也在逐渐改变和完善,这里仅介绍高通量筛选方面的一些最新进展。
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| 关 键 词： | 定向进化 突变库 高通量筛选 |
| 文章编号： | 1672-3678（2006）01-0009-07 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-01-05 |
Research in directed evolution of enzymes |
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| LUO Dian-hui,FANG Bai-shan.Research in directed evolution of enzymes[J].Chinese Journal of Bioprocess Engineering,2006,4(1):9-15. |
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| Authors: | LUO Dian-hui FANG Bai-shan |
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| Institution: | Institute of Industrial Biotechnology, Huaqiao University, Quanzhou 362021, China |
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| Abstract: | Directed evolution has been widely used to change the characteristics of enzyme.The feasible methods for generating gene libraries such as ADO and SHIPREC were introduced in this article;and recent developments in high-throughput screen(HTS) for enzyme activity were discussed. |
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| Keywords: | directed evolution mutant library high-throughput screen(HTS) |
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