Isolation & characterization of Hoechst(low) CD45(negative) mouse lung mesenchymal stem cells |
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Authors: | Chow Kelsey S Jun DuHyun Helm Karen M Wagner David H Majka Susan M |
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Institution: | Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, USA. |
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Abstract: | Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor formation. Taken together these studies suggest that resident lung MSC play a role during pulmonary tissue homeostasis, injury and repair during diseases such as pulmonary fibrosis (PF) and arterial hypertension (PAH). Here we describe a technology to define a population of resident lung MSC. The definition of this population in vivo pulmonary tissue using a define set of markers facilitates the repeated isolation of a well-characterized stem cell population by flow cytometry and the study of a specific cell type and function. |
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Keywords: | Developmental Biology Issue 56 mesenchymal stem cell MSC lung stem cell resident lung mesenchymal stem cells stem cells |
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