| 人血清白蛋白融合技术研究进展 |
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| 引用本文： | 刘文慧,吴敏,沈其,徐迎春,陈枢青.人血清白蛋白融合技术研究进展[J].微生物学杂志,2015(3). |
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| 作者姓名： | 刘文慧 吴敏 沈其 徐迎春 陈枢青 |
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| 作者单位： | 1. 浙江大学药学院生化药学研究室，浙江 杭州 310058；2.浙江海正药业有限公司，浙江 台州318000；3.休斯顿医学研究所，休斯顿，77030,1. 浙江大学药学院生化药学研究室，浙江 杭州 310058；2.浙江海正药业有限公司，浙江 台州318000；3.休斯顿医学研究所，休斯顿，77030,1. 浙江大学药学院生化药学研究室，浙江 杭州 310058；2.浙江海正药业有限公司，浙江 台州318000；3.休斯顿医学研究所，休斯顿，77030,1. 浙江大学药学院生化药学研究室，浙江 杭州 310058；2.浙江海正药业有限公司，浙江 台州318000；3.休斯顿医学研究所，休斯顿，77030,1. 浙江大学药学院生化药学研究室，浙江 杭州 310058；2.浙江海正药业有限公司，浙江 台州318000；3.休斯顿医学研究所，休斯顿，77030 |
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| 摘 要： | 结合笔者所在课题组的实验研究成果，综述人血清白蛋白融合技术研究进展。人血清白蛋白融合技术是近些年来应用较多的用
于改造小分子蛋白和多肽药物的手段。但是众多的研究结果发现将小分子蛋白/多肽和人血清白蛋白融合后会出现一些共性的问题，例
如目标蛋白产率低、易降解等。这些共性问题可以通过融合蛋白的优化设计、蛋白酶缺陷宿主的构建、融合基因多拷贝以及与分子伴侣
共表达得以解决
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| 关 键 词： | 人血清白蛋白融合技术 共性问题 优化设计 蛋白酶缺陷宿主 多拷贝 分子伴侣 |
Research Progress in Fusion Technique of Human Serum Albumin |
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| LIU Wenhui,WU Min,SHEN Qi,XU Yingchun and CHEN Shuqing.Research Progress in Fusion Technique of Human Serum Albumin[J].Journal of Microbiology,2015(3). |
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| Authors: | LIU Wenhui WU Min SHEN Qi XU Yingchun and CHEN Shuqing |
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| Institution: | 1. Department of Biochemical Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Hisun
Pharmaceutical Co., Ltd., Taizhou 318000, China; 3. Houston Methodist Research Institute, Houston 77030, America,1. Department of Biochemical Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Hisun
Pharmaceutical Co., Ltd., Taizhou 318000, China; 3. Houston Methodist Research Institute, Houston 77030, America,1. Department of Biochemical Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Hisun
Pharmaceutical Co., Ltd., Taizhou 318000, China; 3. Houston Methodist Research Institute, Houston 77030, America,1. Department of Biochemical Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Hisun
Pharmaceutical Co., Ltd., Taizhou 318000, China; 3. Houston Methodist Research Institute, Houston 77030, America and 1. Department of Biochemical Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Hisun
Pharmaceutical Co., Ltd., Taizhou 318000, China; 3. Houston Methodist Research Institute, Houston 77030, America |
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| Abstract: | The research progress in fusion technique of human serum albumin (HSA) was reviewed in this paper. Nowadays HSA fusion technique
is a common and effective method for reforming small-molecule protein or peptide drugs. Many studies have shown that there are some common
problems in the fusion of small molecule proteins or peptides with HSA, such as low yield and degradation of the target protein. However, these
problems can be addressed by optimized design of the fusion protein, construction of protease-deficient hosts, multiple copies of fused genes and co-expression with chaperones. |
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| Keywords: | human serum albumin fusion technique common problems optimized design protease-deficient host multiple copies chaperones |
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