| 柱层析法制备乙型脑炎纯化疫苗的研究 |
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| 引用本文： | 宋宗明,岳立广,钱依群,刘双军,李彤,赵红丽,洪成龙,韩亮.柱层析法制备乙型脑炎纯化疫苗的研究[J].微生物学免疫学进展,2004,32(3):9-11. |
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| 作者姓名： | 宋宗明 岳立广 钱依群 刘双军 李彤 赵红丽 洪成龙 韩亮 |
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| 作者单位： | 长春生物制品研究所,长春,130062 |
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| 摘 要： | 为制备纯化乙型脑炎灭活疫苗，以地鼠肾细胞培养并经灭活的乙型脑炎病毒液，浓缩后上Sepharose 4FF凝胶层析柱，用紫外线280nm波长检测得到三个吸收峰。ELISA法证实第一峰为病毒抗原峰，另两个峰为杂蛋白峰。试验证明Seoharose 4FF凝胶过滤对于提纯乙型脑炎病毒是有效的，能去除99％的杂蛋白。
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| 关 键 词： | 乙型脑炎 柱层析 纯化 疫苗制剂 |
| 文章编号： | 1005-5673(2004)03-0009-03 |
Purification of Japanese encephalitis vaccine by column chromatography |
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| SONG Zong-ming,YUE Li-guang,QIAN Yi-qun,et al..Purification of Japanese encephalitis vaccine by column chromatography[J].Progress In Microbiology and Immunology,2004,32(3):9-11. |
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| Authors: | SONG Zong-ming YUE Li-guang QIAN Yi-qun |
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| Abstract: | In order to purify Japanese encephalitis virus,which were cultured in golden hamster kidney cell(GHKC)culture,the culture is purified by Sepharose 4FF gel column chromatography after inactivation and concentration.In this process,three peaks were observed on the profile at a wavelength of 280 nm.The viral antigen was onlydetected out in the first peak by ELISA,the protein impurities were detected out in other peaks.It is a highly efficient method for purification Japanese encephalitis vaccine by Sepharode 4FF gel chromatography,99%protein impurfies can be removed. |
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| Keywords: | Japanese encephalitis Column chromatography Purification Vaccine priparation |
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