Gelatin-entrapped whole-cell invertase

Summary The investigated biocatalyst consists of gelatin-entrapped cells of Saccharomyces cerevisiae retaining invertase activity. Comparative examination of pH profile, apparent K_m, saturation velocity and activation energy indicates that the entrapment procedure did not influence invertase affinity with sucrose but lead to some loss of activity probably due to either enzyme inactivation or cell-wall impairment as well as to substrate diffusion limitation in the gel matrix.