Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana) |
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Authors: | Gondo Takahiro Matsumoto Jun Tsuruta Shin-ichi Yoshida Midori Kawakami Akira Terami Fumihiro Ebina Masumi Yamada Toshihiko Akashi Ryo |
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Institution: | Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan. |
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Abstract: | Rhodes grass (Chloris gayana) is one of the most important warm-season forage grasses. It is cultivated in tropical and subtropical parts of the world and is mostly used for grazing and hay production. We have established a particle-bombardment transformation protocol for rhodes grass using multiple-shoot clumps (MSCs) as the target tissue. A vector pAHC25 containing a herbicide-resistance gene (bar) together with the beta-glucuronidase (GUS) gene was used in transformation experiments. The most efficient recovery of bialaphos-resistant tissue was achieved when the bombarded MSCs were first cultured for 15 d on bialaphos-free medium before being subjected to selection pressure. The resistant tissues regenerated transgenic plants that displayed GUS gene expression. Under optimized conditions, 251 target pieces yielded 46 transgenic plants from 4 independent transgenic lines. |
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