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Molecular engineering of industrial enzymes: recent advances and future prospects |
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| Authors: | Haiquan Yang Jianghua Li Hyun-dong Shin Guocheng Du Long Liu Jian Chen |
| Affiliation: | 1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China 2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China 3. Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, China 4. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, 30332, USA 5. National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China |
| Abstract: | Many enzymes are efficiently produced by microbes. However, the use of natural enzymes as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability, especially under industrial conditions. Many protein engineering technologies have been developed to modify natural enzymes and eliminate these limitations. Commonly used protein engineering strategies include directed evolution, site-directed mutagenesis, truncation, and terminal fusion. This review summarizes recent advances in the molecular engineering of industrial enzymes and discusses future prospects in this field. We expect this review to increase interest in and advance the molecular engineering of industrial enzymes. |
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