Immobilization of glucooligosaccharide oxidase of Acremonium strictum for oligosaccharic acid production |
| |
| Authors: | Shuen-Fuh Lin Ying-Long Hwang Ying-Chieh Tsai |
| |
| Institution: | (1) Department of Bioengineering, Tatung Institute of Technology, Taipei, Taiwan, R.O.C.;(2) Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, R.O.C. |
| |
| Abstract: | Summary The glucooligosaccharide oxidase was covalently immobilized to chitosan with polyethyleneimine and glutaraldehyde. Immobilization improved thermal stability. When used for conversion of starch hydrolysate to oligosaccharic acids, the immobilized enzyme maintained 75% initial activity after 60 days of continuous operation. Strong substrate inhibition was seen at high concentrations of cellobiose and lactose for free enzyme but not for immobilized enzyme. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
