经脂多糖（LPS）处理免疫应答大鼠离体脑片视上核神经元对细胞因？…

实验采用离体脑片全细胞膜片箝记录方法,观察了细胞因子白介素－１β（ＩＬ－１β）和ＩＬ－２对大鼠离体脑片视上核神经元膜电位及自发放电的影响,以期探明免疫应答大鼠视上核神经元对细胞因子敏感性的变化。结果显示,用１００Ｕ／ｍｌ ＩＬ－１β藻流脑片,正常对照的（ｎ＝１５）和脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈｙａｒｉｄｅ ＬＰＳ）腹腔注射９ｄ的大鼠视上核神经元（ｎ＝２０）超极化,同时伴有自发放电频率的下降;

Alteration of sensitivity of supraoptic nucleus neurons to cytokine in the hypothalamic slices from the rat after lipopolysaccharide injection

Using the whole cell patch clamp technique, the effects of interleukin 1beta (IL-1beta) and IL-2 on the membrane potential and spontaneous discharges in the supraoptic nucleus (SON) neurons from the rat hypothalamic slices in vitro were examined to determine the changes in sensitivity of supraoptic nucleus neurons to cytokine. The results obtained are as follows. IL-1beta (100 U/ml) hyperpolarized the SON neurons from the control rats (n=15) and the rats 9 d after i.p. lipopolysaccharide (LPS) (n=20), and decreased the rate of their spontaneous discharges. When 100 U/ml IL-2 was perfused, most SON neurons (n=14) from the control rats were hyperpolarized and the spontaneous firing decreased, but the rest (n=3) remained unchanged. When IL-2 (100 U/ml) was applied, 19 neurons out of the 45 SON neurons from the rats 9 days after i.p. LPS were depolarized accompanied with an increase of the discharges, 16 did not show significant changes in both membrane potential and spontaneous discharges, the rest 10 were hyperpolarized and their discharges were suppressed. These results show that the sensitivity of part of SON neurons from the immuned rat to cytokine IL-2 was altered, suggesting that IL-2 may be involved in modulation of the activity of SON neurons and thus play a role in modulation of the immune response.