Detection of blood group A-like substance in bacterial and viral vaccines by countercurrent immunoelectrophoresis using Helix pomatia lectin |
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Authors: | L S Oravec C J Lee P A Hoppe C V Santos |
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Affiliation: | 1. Barts Health NHS Trust, London, United Kingdom;2. Bolton NHS Foundation Trust, Greater Manchester, United Kingdom;3. Component Development Laboratory, NHS Blood and Transplant, Cambridge, United Kingdom;4. Red Cell Immunohaematology NHS Blood and Transplant, United Kingdom;5. Blizard Institute, Queen Mary University of London, London, United Kingdom;6. Systematic Review Initiative, NHS Blood and Transplant, Oxford, United Kingdom;7. Department of Haematology, University of Cambridge, United Kingdom |
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Abstract: | The technique of countercurrent immunoelectrophoresis (CI), using the N-acetyl glucosamine-binding lectin from Helix pomatia, provided a rapid, sensitive, inexpensive, specific and reliable method for assaying blood group A-like substances in both bacterial and viral vaccines. Blood group A-like substance was detected in the pneumococcal polysaccharide vaccine manufactured by Merck Sharp & Dohme up to 1981 and in a staphylococcus vaccine ( Staphage Lysate) manufactured by Delmont Laboratories. Other US licensed vaccines, including diphtheria and tetanus toxoids, pertussis, meningococcal polysaccharide and influenza vaccines, did not contain detectable amounts of this substance. Human anti-A globulins did not provide a satisfactory reagent for the CI assay because they contained precipitating activities to the vaccine components. |
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