| 5S rDNA间隔序列分析和RAPD技术用于鉴定体细胞杂种 |
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| 引用本文： | 赵同金,向凤宁,聂卉,夏光敏,陈惠民. 5S rDNA间隔序列分析和RAPD技术用于鉴定体细胞杂种[J]. 植物研究, 2004, 24(1): 120-124 |
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| 作者姓名： | 赵同金 向凤宁 聂卉 夏光敏 陈惠民 |
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| 作者单位： | 山东大学生命科学学院, 济南 250100 |
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| 摘 要： | 主要对狭叶柴胡(Bupleurum scoronerifolium)与川西獐芽菜(Swerita musstii Franch) 的科间体细胞杂种愈伤组织、小麦(Triticum aestivum)与燕麦(Avena sativa)的族间体细胞杂种愈伤组织及再生植株进行5S rDNA 间隔序列及RAPD 分析鉴定, 确证了它们为体细胞杂种。
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| 关 键 词： | 5S rDNA 间隔序列分析 RAPD 体细胞杂种 |
| 收稿时间： | 2003-02-21 |
| 修稿时间： | 2003-02-21 |
Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis |
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| ZHAO Tong-Jin XIANG Feng-Ning NIE Hui XIA Guang-Min CHEN Hui-min. Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis[J]. Bulletin of Botanical Research, 2004, 24(1): 120-124 |
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| Authors: | ZHAO Tong-Jin XIANG Feng-Ning NIE Hui XIA Guang-Min CHEN Hui-min |
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| Affiliation: | Life school of Shandong University, Jinan 250100 |
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| Abstract: | The present paper described the 5S rDNA spacer sequence and RAPD analysis in the identification of hybrid nature of the calli line from interfamilial somatic hybridization between Bupleurum scoronerifolium and Swerita musstii Franch, the calli line from intertribal somatic hybridization between Avena sativa and Triticum aestivum L. and its regenerated plants. We confirmed that they were somatic hybrids. |
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| Keywords: | 5S rDNA RAPD |
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