The effect of bisulfite modification on the template activity of DNA for DNA polymerase I |
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| Authors: | Kai K Tsuruo T Hayatsu H |
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| Affiliation: | Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan |
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| Abstract: | Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5,6-dihydrouracil-6-sulfonate (U(SO−3)) residues by treatment with bisulfite. The poly(U(SO−3)2, C3) and poly(U(SO−3)9, C1) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO−3) residues in the DNA inactivated it to serve as template for E.coli DNA polymerase I, while the template activity was restored by conversion of the U(SO−3) residues into U. |
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