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Structural elucidation and immuno-stimulating activity of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba |
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| Authors: | XiuJu Du JingSong Zhang Yan Yang QingJiu Tang YanFang Liu Ruixia Hao YingJie Pan |
| Institution: | a College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China b Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, PR China c College of Food Science, Shanghai Ocean University, Shanghai 200090, PR China d College of Agricultural Science, Liaocheng University, Liaocheng, Shandong 252059, PR China |
| Abstract: | A novel acidic heteropolysaccharide (TAPA1) was purified from hot water extracts of Tremella aurantialba fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-500 High-Resolution Chromatography. The heteropolysaccharide had a molecular weight of ca. 1.35 × 106 Da, and a carbohydrate content estimated to be ∼98.7% by the phenol-sulfuric acid method. It was composed mainly of d-mannose, d-xylose, and d-glucuronic acid in the ratio of ca. 5:4:1, along with trace amounts of d-galacturonic acid and d-glucose. Monosaccharide compositional analysis and GC-MS of methylated derivatives, combined with 1H and 13C NMR spectroscopies (including COSY, TOCSY, NOESY, HSQC, and HMBC spectra), revealed TAPA1 to consist of an α-(1→3)-linked mannopyranosyl backbone, partially substituted at position 4 with xylose side chains, and at position 2 with side chains composed of either xylose, mannose, and glucuronic acid or of xylose and mannose. Bioactivity testing showed that TAPA1 stimulated the proliferation of mouse spleen lymphocytes in vitro. |
| Keywords: | Tremella aurantialba Polysaccharide Structural elucidation NMR spectroscopy Immuno-stimulating activity |
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