Interleukin-10 containing normal human serum inhibits granzyme B release but not perforin release from alloreactive and EBV-specific T cell clones |
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Authors: | Nishimura Motoko Sato Hideya Okazaki Hitoshi Satake Masahiro Tadokoro Kenji |
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Affiliation: | a Department of Research and Development, Central Blood Institute, Japanese Red Cross Society, 2-1-67, Tatsumi, Koutou-ku, Tokyo 135-8521, Japan b Tokyo Metropolitan Red Cross Blood Center, 2-1-67, Tatsumi, Koutou-ku, Tokyo 135-8521, Japan |
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Abstract: | Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor, is capable of inhibiting synthesis of pro-inflammatory cytokines like IFNγ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and T helper Type 1 cells. We observed that normal human serum, derived from a healthy individual but containing large amounts of IL-10, inhibited cytotoxic activity and interfered with granzyme B release from alloreactive cytotoxic T cell (CTL) clones in vitro, but did not affect perforin release. The addition of normal human serum containing high levels of anti-IL-10 IgG neutralized the inhibitory effects of IL-10 serum. Moreover, we have identified that cytotoxic activity and granzyme B release from an Epstein-Barr virus (EBV)-specific CTL clone was similarly inhibited in the presence of IL-10 serum, while perforin release was unaffected. Anti-IL-10 IgG serum also appeared to neutralize the inhibitory effect of IL-10 serum on an EBV-specific CTL clone. |
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Keywords: | Interleukin-10 Alloreactive cytotoxic T cell clone Epstein-Barr virus-specific cytotoxic T cell clone Granzyme B Perforin Anti-IL-10 IgG |
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