Structural organization of pBC1, a cryptic plasmid from Bacillus coagulans. |
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| Authors: | E De Rossi A Milano P Brigidi F Bini and G Riccardi |
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| Institution: | Department of Genetics and Microbiology, A. Buzzati Traverso, University of Pavia, Italy. |
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| Abstract: | The complete nucleotide sequence of the Bacillus coagulans plasmid pBC1 was determined. The sequence revealed an open reading frame encoding a polypeptide of 259 amino acids. This open reading frame shows sequence similarity to genes coding for replication-associated proteins in a group of gram-positive bacterial plasmids known to replicate via single-stranded intermediates. A region required for replication in cis, when the intact replicon is supplied in trans, was identified as well. |
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