Nucleotide sequence of the PR-1 gene of Nicotiana tabacum |
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Authors: | M Ohshima M Matsuoka N Yamamoto Y Tanaka Y Kano-Murakami Y Ozeki A Kato N Harada Y Ohashi |
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Affiliation: | National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki, Japan. |
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Abstract: | A gene encoding one of the pathogenesis-related proteins, PR1a, and two related pseudogenes were isolated from Nicotiana tabacum. The cloned PR1a gene (pPR-gamma) and one of the pseudogenes (pPR-alpha) were sequenced and found to have similar structures. The sequence of pPR-gamma was quite similar to that of the cDNA clone of PR1a. The plasmid pPR-gamma did not contain an intron and had a typical promoter sequence in the 5'-flanking region. |
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