Effect of selected compounds on the activity of glutamate dehydrogenase from triticale roots |
| |
Authors: | J Kwinta K Bartoszewicz W Bielawski |
| |
Institution: | (1) Department of Biochemistry, Warsaw Agricultural University, 02-528 Warszawa, Rakowiecka 26/30, Poland |
| |
Abstract: | The influence of selected factors on the activity of highly purified GDH in triticale roots was investigated in vitro. In the presence of 2-ME, NADH-GDH activity increased by 400 %, while NADPH-GDH activity rose by 500 %. No effect of reducing
factors on NAD(P)+-GDH reaction was detected. The sulphydryl groups inhibitors, such as p-chloromercuribenzoate (p-CMB) and iodoacetamide, proved the strongest inhibitors of the aminative reaction. Metal-binding compounds: ethylenediaminetetraacetic
acid disodium salt (EDTA) and Zinkov also considerably inhibited NAD(P)H-GDH activity. Diisopropylfluorophosphate (DFP) and
pepstatin A, the inhibitors specific for -OH serine and COO− aspartic acid groups respectively, caused a non-significant NAD(P)H-GDH activity decrease. Cd2+, Co2+, Hg2+, Mg2+, Pb2+ and Zn2+ ions strongly inhibited the amination reaction, whereas their inhibiting effect upon NAD+-GDH activity was negligible. Among the applied ions, only Ca2+ activated NADH-GDH. |
| |
Keywords: | glutamate dehydrogenase divalent metal ions inhibitors activators Triticale |
本文献已被 SpringerLink 等数据库收录! |
|