Depolarization-evoked release of gamma-hydroxybutyrate from rat brain slices |
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Authors: | M Maitre C Cash D Weissmann-Nanopoulos P Mandel |
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Institution: | Centre de Neurochimie du CNRS and Unité44 de I'INSERM, Strasbourg, France |
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Abstract: | The release of gamma-hydroxybutyrate from preloaded rat brain striatal slices was investigated. K+-induced depolarization caused an efflux of gamma-hydroxybutyrate of about 50 fmol min-1 mg-1 (wet weight), but in a Ca2+-free medium containing Mg2+, the evoked release was reduced by 50-60%. The release was higher when 100 microM veratridine was used as a depolarizing agent. The efflux of gamma-hydroxybutyrate is related to veratridine and K+ concentration, and is strongly inhibited by 10 microM tetrodotoxin. The Ca2+ channel blocker verapamil induces a large decrease in the efflux of gamma-hydroxybutyrate after both K+- and veratridine-induced depolarization. These results are in favour of a possible transmitter function for gamma-hydroxybutyrate in rat striatum. |
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Keywords: | γ-Hydroxybutyrate Release Depolarization Brain slices (rat) |
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