Use of thiopropyl Sepharose for the synthesis of an adsorbent for the affinity chromatography of glutathione S-transferase |
| |
Authors: | Z. Glatz ,J. Psotová ,O. Janiczek,K. Chroust,T. Jowet |
| |
Affiliation: | Z. Glatz, J. Psotová, O. Janiczek, K. Chroust,T. Jowet |
| |
Abstract: | Thiopropyl Sepharose 6B in the 2-thiopyridyl-activated form was used for the reversible immobilisation of reduced glutathione (GSH). The resulting affinity matrix was successfully tested as a sorbent for the partial purification of glutathione S-transferase (GST) from pig kidney. The specific elution of the enzyme was performed with 10 mM GSH in Tris-HCl buffer (pH 7.8), non-specific elution with 20 mM dithiotreitol (DTT) in the same buffer. |
| |
Keywords: | Glutathione S-transferase Enzymes |
本文献已被 ScienceDirect 等数据库收录! |
|