Cytoplasmic acidification and activation of Na+/H+ exchange during regulatory volume decrease in Ehrlich ascites tumor cells |
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Authors: | Avinoam Livne Else K. Hoffmann |
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Affiliation: | (1) Institute of Biological Chemistry A, August Krogh Institute, University of Copenhagen, DK-2100 Copenhagen O, Denmark;(2) Department of Biology, Ben-Gurion University of the Negev, 84105 Beer Sheva, Israel |
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Abstract: | Summary Ehrlich ascites tumor cells undergoing regulatory volume decrease (RVD) exhibit cytoplasmic acidification as measured by an intracellular fluorescent pH indicator. The acidification results in an activation of the Na+/H+ exchanger. The intracellular pH set point for the activation is estimated to be around 7.0. The activation of the Na+/H+ exchanger leads to an incomplete RVD. In support of this conclusion, amiloride and Na+-free medium, known to limit the Na+/H+ exchange, indeed enhance the RVD response. Intracellular acidification and activation of Na+/H+ exchange may be a general response of cells undergoing RVD. |
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Keywords: | Ehrlich ascites tumor cells volume regulation cytoplasmic acidification pH regulation Na+/H+ exchange |
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