High activity extracellular glucose (xylose) isomerase from a Chainia species

Summary A sclerotia-forming actinomycete of the genus Chainia secreted high levels of glucose (xylose) isomerase when grown in submerged culture on a wheat bran - yeast extract medium. Maximum activity (4 units/ml) was obtained after 3–4 days when the cell bound activity was 0.19 units/ml. The two enzymes differed significantly in pH optima (extracellular, 9.5; cell-bound, 7.0) and in their adsorption behaviour on CM and DEAE celluloses. Both Mg⁺⁺ and Co⁺⁺ are required by the cell-bound enzyme for its optimum activity while either Mg⁺⁺ or Co⁺⁺ is necessary for the extracellular enzyme.NCL Communication 3320