Anti-GM3-lactam monoclonal antibodies of the IgG type recognize natural GM3-ganglioside lactone but not GM3-ganglioside |
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Authors: | Kai Ding Anders Rosén Asim K. Ray Göran Magnusson |
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Affiliation: | (1) Hybridoma Group, Department of Medical Cell Genetics, Karolinska Institutet, S-10401 Stockholm, Sweden;(2) Organic Chemistry 2, Chemical Center, The Lund Institute of Technology, University of Lund, Box 124, S-22100 Lund, Sweden |
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Abstract: | Immunization of mice with a synthetic GM3-lactam-BSA (bovine serum albumin) conjugate (designed to emulate the corresponding natural GM3-lactone conjugate), followed by fusion of splenocytes with myeloma cells, gave rise to more than 300 monoclonal hybridomas producing antibodies to GM3-lactam-BSA, which did not react with Glc-BSA and BSA. Eight antibody clones were randomly chosen from the positive 300 hybridomas. The eight clones, all belonging to the IgG class, were unreactive against GM3-ganglioside, whereas two antibodies (P5-1 and P5-3, both IgG1, ) reacted with GM3-ganglioside lactone. Binding of these two antibodies to the GM3-lactam-BSA conjugate was inhibited by soluble glycosides of GM2-, GM3-, and GM4-lactam and by GM3- and GM4-lactam, respectively, but not by Gb3 or asialo-GM1 and GM2-saccharides. A third antibody (P3; IgG2b, ) was inhibited by GM2-, GM3-, and GM4-lactam, but did not recognize GM3-ganglioside lactone. |
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Keywords: | anti-GM3-lactam and anti-GM3-lactone monoclonal IgG antibodies |
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