Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates

Authors:	Jari?Rautio,Kim?Bundvig?Barken,Juhani?Lahdenper?,Antje?Breitenstein,S?ren?Molin,Peter?Neubauer author-information" > author-information__contact u-icon-before" > mailto:peter.neubauer@oulu.fi" title=" peter.neubauer@oulu.fi" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Finland;(2) Novozymes A/S, Smoermosevej 25, DK2880 Bagsvaerd, Denmark;(3) BioCentrum-DTU, The Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark

Abstract:	Background A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. The 18S rRNA and SUC2 mRNA of Saccharomyces cerevisiae were used as model RNA target molecules.

Keywords:
本文献已被 PubMed SpringerLink 等数据库收录！