Alternative method for rapidly screening microbial isolates for their potential to degrade volatile contaminants |
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Authors: | Strong-Gunderson Janet M Palumbo Anthony V |
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Institution: | (1) Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, 37831-6038 Oak Ridge, TN, USA |
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Abstract: | Summary A method is described for rapidly screening the metabolic potential of bacteria to oxidize semivolatile and volatile compounds as a sole carbon source. The method is based on automated system that utilizes MicroplatesTM manufactured by Biolog, Inc. (Hayward, CA, USA). This system detects bacterial respiratory activity from the oxidation of a carbon source introduced in volatile form. This is in contrast to the original design, which is based on inoculating a carbon source directly into each well. The 96-well (MT) microtiter plates contain nutrients and a tetrazolium dye. When a bacterial species is capable of oxidizing a volatile carbon substrate, the dye turns purple, and a spectrophotometric plate reader quantifies the response. As a test of this method 150 isolates, including isolates known to degrade some of the test compounds and negative controls were evaluated for their potential to oxidize carbon tetrachloride, toluene, ando-xylene. Thirty-seven isolates (25%) were qualitatively identified as contaminant oxidizers, and thirteen of these (35%) showed significant degradation capabilities for both toluene ando-xylene.Environmental Sciences Division Publication Number 4277. |
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Keywords: | Bioremediation Volatile organic contaminants Carbon tetrachloride Toluene o-xylene |
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