Identification of a fibrinolytic enzyme by <Emphasis Type="Italic">Bacillus vallismortis</Emphasis> and its potential as a bacteriolytic enzyme against <Emphasis Type="Italic">Streptococcus mutans</Emphasis> |
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Authors: | Jae-Bum Kim Woo-Hyuk Jung Ji-Myung Ryu Yeo-Joon Lee Joon-Ki Jung Hyung-Wook Jang Sung-Woo Kim |
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Institution: | (1) AceBiotech Co., Ltd., #114 Bio-Venture Center, KRIBB, Yuseong, Daejeon, 305-806, Korea;(2) Division of Biotechnology R&BD, KRIBB, Yuseong, Daejeon, 305-806, Korea |
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Abstract: | A potent fibrinolytic enzyme-producing bacterium was isolated from the traditional Korean condiment Chungkook-jang and identified
as Bacillus vallismortis Ace02. The extracellular fibrinolytic enzyme was purified with a 18% recovery of activity from supernatant cultures using
CM-Sepharose column chromatography and Sephacryl S-200 gel filtration. The specific activity of the purified enzyme was 757 kFU mg−1. Its molecular mass was about 28 kDa and the initial amino acids of the N-terminal sequence were AQSVPYGVSQ. The full amino acid sequence of fibrinolytic enzyme Ace02 corresponded with bacteriolytic
enzyme, L27, from Bacillus licheniformis, which has strong lytic activity against Streptococcus mutans, a major causative strain of dental caries. This suggests that the purified enzyme should be used for prevention of dental
caries as well as being an effective thrombolytic agent. |
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Keywords: | Bacillus vallismortis Ace02 Bacteriolytic enzyme Fibrinolytic enzyme |
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