Newly administered [3H]retinol is transferred from hepatocytes to stellate cells in liver for storage |
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Authors: | R Blomhoff K Holte L Naess T Berg |
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Institution: | 1. Department of Cell Growth and Regulation, Dana-Farber Cancer Institute USA;2. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA |
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Abstract: | We have recently shown that newly administered vitamin A (retinol) is initially taken up by the parenchymal cells of the liver, and subsequently (within 1-2 h) transferred to non-parenchymal liver cells (NPC) (Blomhoff et al., ref. 10]). In the present study we have separated the NPC by different methods to determine the cell type responsible for this uptake of 3H]retinol. When liver cells were prepared between 5 and 18 h after intraduodenal administration of 3H]retinol, the radioactive retinol was recovered mainly in the stellate cells. Other liver cells (i.e., hepatocytes, endothelial cells and Kupffer cells) contained only small amounts of 3H]retinol. Further, fluorescence microscopy studies indicated that stellate cells contain large quantities of retinol. Our results show that newly administered 3H]retinol, which is initially located in the hepatocytes, is transferred to the stellate cells and stored there. |
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