| 胸腺五肽纳滤浓缩过程研究 |
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| 引用本文： | 李鑫,欧阳嘉,徐勇,陈牧,宋向阳,应汉杰.胸腺五肽纳滤浓缩过程研究[J].生物加工过程,2009,7(2):39-43. |
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| 作者姓名： | 李鑫 欧阳嘉 徐勇 陈牧 宋向阳 应汉杰 |
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| 作者单位： | 1. 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,南京,210037
2. 南京工业大学,制药与生命科学学院,南京,210009 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展规划(973计划) |
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| 摘 要： | 采用间歇浓缩方式，研究了纳滤对胸腺五肽离子交换洗脱液的浓缩特性。采用纳滤浓缩模型预测胸腺五肽离子交换洗脱液纳滤过程，系统考察透过通量和胸腺五肽浓度等随过程时间的变化。实验结果表明：截留相对分子质量为150的纳滤膜对胸腺五肽的截留率达到98．66％；胸腺五肽洗脱液透过通量随操作压力变化的结果表明，其纳滤过程为两机理控制；纳滤浓缩模型较好地模拟了胸腺五肽的纳滤浓缩过程，说明该模型适用于小分子多肽的纳滤浓缩过程。
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| 关 键 词： | 纳滤 间歇过程 胸腺五肽 浓缩 |
Nanofiltration concentration of thymopentin |
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| LI Xin,OUYANG Jia,XU Yong,CHEN Mu,SONG Xiang-yang,YING Han-jie.Nanofiltration concentration of thymopentin[J].Chinese Journal of Bioprocess Engineering,2009,7(2):39-43. |
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| Authors: | LI Xin OUYANG Jia XU Yong CHEN Mu SONG Xiang-yang YING Han-jie |
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| Institution: | 1. Key Laboratory of Forest Genetics ＆ Biotechnology, Ministry of Education,Nanjing Forestry University,Nanjing 210037,China ; 2. College of Life Science and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | The batch concentration of thymopentin solution from ion-exchange process by nanofiltration was investigated. The selectivity of nanofihration membrane, the permeation flux,and the concentration efficiency were discussed. The retention ratio of thymopentin was 98. 66% with the nanofiltration membrane of molecular weight cut-off of 150. The result showed that a bi-control mechanism of nanofiltration resulted from the permeation flux changed with the applied pressures. The concentration model could be used to describe the procedure of nanofiltration of thymopentin solution from ion-exchange. The model is changed with process time, thus it was suitable for designing the process of small molecule synthetic peptides. |
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| Keywords: | nanofiltration batch process thymopentin concentration |
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