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A genetic diversity study of silkworm using cleaved amplified polymorphic sequence (CAPS) markers |
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| Authors: | Jianhua Huang Muwang Li Yong Zhang Wenbin Liu Minghui Li Xuexia Miao Yongping Huang |
| Affiliation: | aInstitute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Feng Lin Road, Shanghai 200032, PR China bGraduate School of the Chinese Academy of Sciences, Beijing 100039, PR China |
| Abstract: | The silkworm, Bombyx mori is a beneficial insect of great economic importance in China for its silk production. In this study, we obtained 11 cleaved amplified polymorphic sequence (CAPS) markers and one PCR polymorphism marker from the genes of the silkworm, B. mori. A backcross progeny analysis showed that all these molecular markers were segregated in a Mendelian fashion and that polymorphisms were co-dominant. These markers were used to investigate the genetic diversity among 29 strains of B. mori from China, Japan and Europe. Cluster analysis, based on the genetic similarities calculated from CAPS data, grouped these strains roughly according to their geographical origin. One group contained silkworm strains from Europe and some of the Japanese strains were interspersed into the Chinese groups, whereas other Japanese strains clustered together. |
| Keywords: | Silkworm Bombyx mori CAPS markers Polymorphism Genetic diversity Geographical Restriction endonuclease |
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