| 蛋白激酶C对大鼠支气管平滑肌KV通道的影响 |
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| 引用本文： | Liu XS,Xu YJ,Zhang ZX,Ni W,Chen SX. 蛋白激酶C对大鼠支气管平滑肌KV通道的影响[J]. 生理学报, 2003, 55(2): 135-141 |
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| 作者姓名： | Liu XS Xu YJ Zhang ZX Ni W Chen SX |
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| 作者单位： | 华中科技大学同济医学院附属同济医院呼吸内科,武汉,430030 |
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| 基金项目： | ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 30 2 70 5 83) |
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| 摘 要： | 用全细胞膜片钳、Western印迹法和逆转录—PCR技术,观察蛋白激酶C(protein kinase C,PKC)对大鼠支气管平滑肌细胞(bronchial smooth muscle cells,BSMCs)电压依赖性延迟整流钾通道(Kv)活性及其亚型Kvl．5表达的影响。结果为：(1)PKC激活剂豆蔻酰佛波醇乙酯(phorbol 12-myristate 13-acetate,PMA)显著抑制急性分离大鼠BSMCs的Kv通道电流,该效应被PKC阻断剂Ro31—8220显著抑制;(2)PMA显著抑制体外培养大鼠BSMCs的Kvl．5 mRNA和蛋白质的表达,该效应被Ro31—8220显著抑制。上述观察结果提示,PKC活化可抑制大鼠BSMCs的Kv通道电流活性,下调Kvl．5亚型的表达水平。
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| 关 键 词： | 支气管 平滑肌细胞 电压依赖性延迟整流钾通道 蛋白激酶C |
| 修稿时间： | 2002-07-01 |
Effect of protein kinase C on K(V) channel in rat bronchial smooth muscle |
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| Liu Xian-Sheng,Xu Yong-Jian,Zhang Zhen-Xiang,Ni Wang,Chen Shi-Xin. Effect of protein kinase C on K(V) channel in rat bronchial smooth muscle[J]. Acta Physiologica Sinica, 2003, 55(2): 135-141 |
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| Authors: | Liu Xian-Sheng Xu Yong-Jian Zhang Zhen-Xiang Ni Wang Chen Shi-Xin |
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| Affiliation: | Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030; E-mail: liuxiansheng@tih.tjmu.edu.cn |
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| Abstract: | The effect of protein kinase C (PKC) signaling pathway on the activity of voltage-dependent delayed rectifier potassium channel (K V) and the expression of K V isoform K V1 5 in rat bronchial smooth cells (BSMCs) were investigated with whole-cell patch clamp, Western-blot and RT-PCR techniques. The results showed: (1) phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a significant inhibition of K V channel currents in rat BSMCs. The inhibition was partly abolished by Ro31-8220, a PKC inhibitor. (2) PMA caused a significant suppression of the expression of K V1 5 mRNA and protein in rat BSMCs. These effects were attenuated by Ro31-8220. The results suggest that in rat BSMCs PKC activation inhibits K V currents and down-regulates the expression of K V1 5. |
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| Keywords: | bronchus smooth muscle cells voltage-dependent delayed rectifier potassium channel protein kinase C |
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